Yong Hyun Chung, Jeong Hee Han, Sung-Bae Lee and Yong-Hoon Lee. Inhalation Toxicity of Bisphenol A and Its Effect on Estrous Cycle, Spatial Learning, and Memory in Rats upon Whole-Body Exposure. Toxicol. Res. 2017; 33(2): 165-171

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双酚A的吸入毒性及其对全身暴露大鼠发情周期、空间学习和记忆能力的影响

摘要：双酚A（BPA）作为聚合反应单体被用于生产聚碳酸酯塑料。它在许多消费品中被使用，包括塑料、聚氯乙烯、食品包装、牙科密封剂和热收据等等。然而，关于双酚A（BPA）的吸入毒性报道很少。因此，本研究的目的是确定其吸入毒性对全身暴露大鼠发情周期、空间学习和记忆能力的影响。通过全身吸入药剂方法，将Sprague-Dawley大鼠暴露于0,10,30和90mg/m3BPA环境中，6小时/天，5天/周，持续8周。比较了对照组和BPA暴露组之间的死亡率、临床症状、体重、血液学、血清化学、发情周期参数、Morris水迷宫试验的表现、器官重量以及总体和组织病理学损伤结果。观察到BPA暴露组的血清化学和器官重量发生统计学意义上显着的变化。然而，BPA暴露组的雄性或雌性大鼠的体重、食物消耗、血液学、血清化学、器官重量、发情周期、Morris水迷宫试验中的表现以及总体或组织病理学损伤方面，没有观察到BPA的毒性作用。总之，本研究的结果表明，BPA对大鼠的无可见有害作用水平（NOAEL）高于90mg/m3/6小时/天，5天/周，持续8周暴露。不仅如此，BPA不影响大鼠的发情周期、空间学习或记忆能力。

关键词：双酚A（BPA），无可见有害作用水平，全身吸入毒性，发情周期，Morris水迷宫测试，内分泌干扰物化学品

Inhalation Toxicity of Bisphenol A and Its Effect on Estrous Cycle, Spatial Learning, and Memory in Rats upon Whole-Body Exposure

ABSTRACT：Bisphenol A (BPA) is a monomer used in a polymerization reaction in the production of polycarbonate plastics. It has been used in many consumer products, including plastics, polyvinyl chloride, food packaging, dental sealants, and thermal receipts. However, there is little information available on the inhalation toxicity of BPA. Therefore, the aim of this study was to determine its inhalation toxicity and effects on the estrous cycle, spatial learning, and memory. Sprague-Dawley rats were exposed to 0, 10, 30, and 90 mg/m3 BPA, 6 hr/day, 5 days/week for 8 weeks via whole-body inhalation. Mortality, clinical signs, body weight, hematology, serum chemistry, estrous cycle parameters, performance in the Morris water maze test, and organ weights, as well as gross and histopathological findings, were compared between the control and BPA exposure groups. Statistically significant changes were observed in serum chemistry and organ weights upon exposure to BPA. However, there was no BPA-related toxic effect on the body weight, food consumption, hematology, serum chemistry, organ weights, estrous cycle, performance in the Morris water maze test, or gross or histopathological lesions in any male or female rats in the BPA exposure groups. In conclusion, the results of this study suggested that the no observable adverse effect level (NOAEL) for BPA in rats is above 90 mg/m3/6 hr/day, 5 days/week upon 8-week exposure. Furthermore, BPA did not affect the estrous cycle, spatial learning, or memory in rats.

Key words: Bisphenol A, NOAEL, Whole-body inhalation toxicity, Estrous cycle, Morris water maze test, Endocrine disruptor chemicals